cfx96 real time pcr detection system (Bio-Rad)
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cfx96 real time pcr detection system
Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/real+time+pcr+detection+system/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc13091180-120-14-19
Average 96 stars, based on 27901 article reviews
Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/real+time+pcr+detection+system/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc13091180-120-14-19
Average 96 stars, based on 27901 article reviews
cfx96 real time pcr detection system - by Bioz Stars,
2026-09
96/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Gene expression and histological assessment of capsular fibrosis in post-traumatic shoulder stiffness following plate fixation of proximal humeral fractures: an exploratory pilot study. Article Snippet: Subsequently RNA was reverse transcribed into cDNA with the Transcriptor First Strand cDNA Synthesis kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions. .. Quantitative real-time polymerase chain reaction (PCR) was performed using a customized 96-wells plate (PrimePCRTM Primers, Bio-Rad Laboratories, Hercules, CA, USA) and a Article Title: Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity Article Snippet: Sypro Orange 5000X (Sigma) was added to the protein solution to reach a final concentration of 5X, and the mixtures were transferred to a skirted 96-well PCR plate. .. The assay was conducted using a Article Title: Defective branched-chain amino acid catabolism promotes pulmonary fibrosis by inducing apoptosis resistance of myofibroblasts in mice. Article Snippet: Real-time PCR was performed using SYBR green master mix (Takara). .. The fluorescence spectra were monitored using a CFX-96 Article Title: Extracellular Vesicle from Chlorella vulgaris Alleviates Hepatic Fibrosis in a Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease Through Modulation of Inflammatory Signaling. Article Snippet: The total RNA was extracted using ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan). .. RNA purity and concentration were determined with a Nanodrop spectrophotometer (Thermo Fisher Scientific K.K., Tokyo, Japan) by calculating the absorbance ratio at 260/280 nm. cDNA was synthesized by mixing purified RNA with 50 ng/μL oligo d(T) and 1 mM of dNTPs and incubating the mixture for 5 min at 65 ◦C, followed by reverse transcription in RT buffer (50 mM of Tris-HCl at pH 8.3, 75 mM of KCl, 3 mM MgCl2, and 5 mM of dithiothreitol) containing 50 U of SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and 20 U of RNaseOUT RNase inhibitor (Invitrogen) at 50 ◦C for 60 min. qPCR was performed using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Osaka, Japan) and gene-specific primers (Table S2) on a Bio-Rad CFX Connect Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair Article Snippet: For qRT-PCR, total RNA was extracted from BMSCs using TRIzol reagent according to the manufacturer's instructions and quantified with a spectrophotometer (NanoDrop 2000, Thermo Scientific, USA). cDNA synthesis was performed using 1 μg of total RNA with the PrimeScript RT Reagent Kit. .. Amplification reactions were conducted with SYBR Greenon a Article Title: A new family of rolling-circle replication endonucleases widespread in archaeal viruses and plasmids Article Snippet: The qPCR reaction was performed in a 20 μl mixture containing 5 μl of template DNA, 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad), 1 μl of primer pairs (10 μM), and 4 μl of distilled water. .. The amplification was carried out on a CFX96 Connect Article Title: Chloroplast stress caused by maltose hyperaccumulation activates chlorophagy via the core autophagy machinery. Article Snippet: Equal amounts of total RNA were subjected to reverse transcription 20 with PrimeScript RT Master Mix (Takara). .. An aliquot of the first-strand cDNA was subjected 21 to qPCR analysis using standard curves with SsoAdvanced Universal SYBR Green Supermix 22 (Bio-Rad) on a Article Title: Transcriptome profiling to decipher molecular mechanisms of diapause in Chilo partellus (Swinhoe) (Lepidoptera: Crambidae). Article Snippet: Diapause is a critical adaptive strategy in insects to survive unfavourable environmental conditions.. The present study investigated molecular mechanisms underlying diapause in Chilo partellus, using high-throughput RNA sequencing and de novo transcriptome assembly.. Six transcriptome libraries from diapause and non-diapause larvae yielded over 50 million reads per sample, with >98% mapping efficiency. Polymerase Chain Reaction:Article Title: Gene expression and histological assessment of capsular fibrosis in post-traumatic shoulder stiffness following plate fixation of proximal humeral fractures: an exploratory pilot study. Article Snippet: Subsequently RNA was reverse transcribed into cDNA with the Transcriptor First Strand cDNA Synthesis kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions. .. Quantitative real-time polymerase chain reaction (PCR) was performed using a customized 96-wells plate (PrimePCRTM Primers, Bio-Rad Laboratories, Hercules, CA, USA) and a Fluorescence:Article Title: Defective branched-chain amino acid catabolism promotes pulmonary fibrosis by inducing apoptosis resistance of myofibroblasts in mice. Article Snippet: Real-time PCR was performed using SYBR green master mix (Takara). .. The fluorescence spectra were monitored using a CFX-96 Concentration Assay:Article Title: Extracellular Vesicle from Chlorella vulgaris Alleviates Hepatic Fibrosis in a Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease Through Modulation of Inflammatory Signaling. Article Snippet: The total RNA was extracted using ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan). .. RNA purity and concentration were determined with a Nanodrop spectrophotometer (Thermo Fisher Scientific K.K., Tokyo, Japan) by calculating the absorbance ratio at 260/280 nm. cDNA was synthesized by mixing purified RNA with 50 ng/μL oligo d(T) and 1 mM of dNTPs and incubating the mixture for 5 min at 65 ◦C, followed by reverse transcription in RT buffer (50 mM of Tris-HCl at pH 8.3, 75 mM of KCl, 3 mM MgCl2, and 5 mM of dithiothreitol) containing 50 U of SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and 20 U of RNaseOUT RNase inhibitor (Invitrogen) at 50 ◦C for 60 min. qPCR was performed using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Osaka, Japan) and gene-specific primers (Table S2) on a Bio-Rad CFX Connect Spectrophotometry:Article Title: Extracellular Vesicle from Chlorella vulgaris Alleviates Hepatic Fibrosis in a Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease Through Modulation of Inflammatory Signaling. Article Snippet: The total RNA was extracted using ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan). .. RNA purity and concentration were determined with a Nanodrop spectrophotometer (Thermo Fisher Scientific K.K., Tokyo, Japan) by calculating the absorbance ratio at 260/280 nm. cDNA was synthesized by mixing purified RNA with 50 ng/μL oligo d(T) and 1 mM of dNTPs and incubating the mixture for 5 min at 65 ◦C, followed by reverse transcription in RT buffer (50 mM of Tris-HCl at pH 8.3, 75 mM of KCl, 3 mM MgCl2, and 5 mM of dithiothreitol) containing 50 U of SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and 20 U of RNaseOUT RNase inhibitor (Invitrogen) at 50 ◦C for 60 min. qPCR was performed using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Osaka, Japan) and gene-specific primers (Table S2) on a Bio-Rad CFX Connect Synthesized:Article Title: Extracellular Vesicle from Chlorella vulgaris Alleviates Hepatic Fibrosis in a Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease Through Modulation of Inflammatory Signaling. Article Snippet: The total RNA was extracted using ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan). .. RNA purity and concentration were determined with a Nanodrop spectrophotometer (Thermo Fisher Scientific K.K., Tokyo, Japan) by calculating the absorbance ratio at 260/280 nm. cDNA was synthesized by mixing purified RNA with 50 ng/μL oligo d(T) and 1 mM of dNTPs and incubating the mixture for 5 min at 65 ◦C, followed by reverse transcription in RT buffer (50 mM of Tris-HCl at pH 8.3, 75 mM of KCl, 3 mM MgCl2, and 5 mM of dithiothreitol) containing 50 U of SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and 20 U of RNaseOUT RNase inhibitor (Invitrogen) at 50 ◦C for 60 min. qPCR was performed using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Osaka, Japan) and gene-specific primers (Table S2) on a Bio-Rad CFX Connect Purification:Article Title: Extracellular Vesicle from Chlorella vulgaris Alleviates Hepatic Fibrosis in a Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease Through Modulation of Inflammatory Signaling. Article Snippet: The total RNA was extracted using ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan). .. RNA purity and concentration were determined with a Nanodrop spectrophotometer (Thermo Fisher Scientific K.K., Tokyo, Japan) by calculating the absorbance ratio at 260/280 nm. cDNA was synthesized by mixing purified RNA with 50 ng/μL oligo d(T) and 1 mM of dNTPs and incubating the mixture for 5 min at 65 ◦C, followed by reverse transcription in RT buffer (50 mM of Tris-HCl at pH 8.3, 75 mM of KCl, 3 mM MgCl2, and 5 mM of dithiothreitol) containing 50 U of SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and 20 U of RNaseOUT RNase inhibitor (Invitrogen) at 50 ◦C for 60 min. qPCR was performed using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Osaka, Japan) and gene-specific primers (Table S2) on a Bio-Rad CFX Connect Reverse Transcription:Article Title: Extracellular Vesicle from Chlorella vulgaris Alleviates Hepatic Fibrosis in a Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease Through Modulation of Inflammatory Signaling. Article Snippet: The total RNA was extracted using ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan). .. RNA purity and concentration were determined with a Nanodrop spectrophotometer (Thermo Fisher Scientific K.K., Tokyo, Japan) by calculating the absorbance ratio at 260/280 nm. cDNA was synthesized by mixing purified RNA with 50 ng/μL oligo d(T) and 1 mM of dNTPs and incubating the mixture for 5 min at 65 ◦C, followed by reverse transcription in RT buffer (50 mM of Tris-HCl at pH 8.3, 75 mM of KCl, 3 mM MgCl2, and 5 mM of dithiothreitol) containing 50 U of SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and 20 U of RNaseOUT RNase inhibitor (Invitrogen) at 50 ◦C for 60 min. qPCR was performed using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Osaka, Japan) and gene-specific primers (Table S2) on a Bio-Rad CFX Connect Amplification:Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair Article Snippet: For qRT-PCR, total RNA was extracted from BMSCs using TRIzol reagent according to the manufacturer's instructions and quantified with a spectrophotometer (NanoDrop 2000, Thermo Scientific, USA). cDNA synthesis was performed using 1 μg of total RNA with the PrimeScript RT Reagent Kit. .. Amplification reactions were conducted with SYBR Greenon a Article Title: A new family of rolling-circle replication endonucleases widespread in archaeal viruses and plasmids Article Snippet: The qPCR reaction was performed in a 20 μl mixture containing 5 μl of template DNA, 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad), 1 μl of primer pairs (10 μM), and 4 μl of distilled water. .. The amplification was carried out on a CFX96 Connect Article Title: Transcriptome profiling to decipher molecular mechanisms of diapause in Chilo partellus (Swinhoe) (Lepidoptera: Crambidae). Article Snippet: Diapause is a critical adaptive strategy in insects to survive unfavourable environmental conditions.. The present study investigated molecular mechanisms underlying diapause in Chilo partellus, using high-throughput RNA sequencing and de novo transcriptome assembly.. Six transcriptome libraries from diapause and non-diapause larvae yielded over 50 million reads per sample, with >98% mapping efficiency. SYBR Green Assay:Article Title: Chloroplast stress caused by maltose hyperaccumulation activates chlorophagy via the core autophagy machinery. Article Snippet: Equal amounts of total RNA were subjected to reverse transcription 20 with PrimeScript RT Master Mix (Takara). .. An aliquot of the first-strand cDNA was subjected 21 to qPCR analysis using standard curves with SsoAdvanced Universal SYBR Green Supermix 22 (Bio-Rad) on a |